辐射源通过转化生长因子-β-介导的上皮-间质转换来促进癌细胞的侵袭迁移

Int J Radiat Oncol Biol Phys 2011 Dec;81 (5): 1530-7. [IF:4.503]Ionizing radiation promotes migration and invasion of cancer cells through transforming growth factor-Beta-mediated epithelial-mesenchymal transition.Zhou YC , Liu JY , Li J , Zhang J , Xu YQ , Zhang HW , Qiu LB , Ding GR , Su XM , Mei-Shi , Guo GZ .Department of Radiation Oncology, Xijing Hospital Fourth Military Medical University, Xi'an, China; Department of Radiation Medicine, College of Preventive Medicine, Xijing Hospital Fourth Military Medical University, Xi'an, China.第四军医大学西京医院放射科

AbstractTo examine whether ionizing radiation enhances the migratory and invasive abilities of cancer cells through transforming growth factor (TGF-β)-mediated epithelial-mesenchymal transition (EMT). Six cancer cell lines originating from different human organs were irradiated by (60)Co γ-ray at a total dose of 2 Gy, and the changes associated with EMT, including morphology, EMT markers, migration and invasion, were observed by microscope, Western blot, immunofluorescence, scratch assay, and transwell chamber assay, respectively. Then the protein levels of TGF-β in these cancer cells were detected by enzyme-linked immunosorbent assay, and the role of TGF-β signaling pathway in the effect of ionizing radiation on EMT was investigate by using the specific inhibitor SB431542. After irradiation with γ-ray at a total dose of 2 Gy, cancer cells presented the mesenchymal phenotype, and compared with the sham-irradiation group the expression of epithelial markers was decreased and of mesenchymal markers was increased, the migratory and invasive capabilities were strengthened, and the protein levels of TGF-β were enhanced. Furthermore, events associated with EMT induced by IR in A549 could be reversed through inhibition of TGF-β signaling. These results suggest that EMT mediated by TGF-β plays a critical role in IR-induced enhancing of migratory and invasive capabilities in cancer cells.

摘要 ：探讨辐射源有否可通过转化激素-β（TGF-β）-激活的上皮-泌尿系统切换 (EMT)来促进癌蛋白的侵袭迁移。使用增幅2Gy(60)Coγ线照射源自人类器官的6种癌蛋白，记录与EMT之外的变化，这包括分别为了让显微镜技术，蛋白质印迹分析方法，免疫荧光技术，污渍试验和Transwell小室试验来注意到并检测蛋白组织形体，EMT标明，侵袭迁移能力等。换用酶联免疫吸附法检测这些癌蛋白中会TGF-β蛋白水平，为了让值得注意类似物SB431542来评估TGF-β瞬时通路在辐射源EMT中会的作用。经过增幅为2Gy照射的癌蛋白中会存有间叶蛋白的表述，与所谓照射组相比之下其上皮标明减少，间叶蛋白标明增加，同时其侵袭移出能力提升，TGF-β蛋白水平也提高。进一步挖掘出由A549辐射源可借的EMT可通过对TGF-β瞬时抑制发生翻盘。这些相比之下较TGF-β激活的EMT在辐射源可借提升癌蛋白侵袭移出能力中会起着关键作用。

编辑： lizexiu